Mycoplasma species Reagent Set

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Code
EMK050050
Category
PCR Kit
Format
50rxns
Description
Reagent Set Mycoplasma
Mycoplasma species Reagent Set
Reagent Set for the detection of Mycoplasma in cell culture supernatant
Mycoplasma can easily contaminate cell cultures. Traditional detection methods (cultural and immunoenzymatic often do not have sufficient sensitivity to detect low grade contaminations) The primers are able to detect all Mycoplasma species and allow a high sensitivity
Starting sample
200µl of growing medium
Primers
Primers are aliquoted in single reaction tubes and isolated by paraffin (HOT-START) Primers contain an UV-activable DNA cross-linker that makes the amplicons not further suitable for PCR (post-amplification decontamination)
Reagents supplied
Sixty tubes containing 20µl of primer solution includes also dNTPs and Thermostable DNA polymerase (red vials). Two tubes containing 60µl of positive control including also dNTPs and Thermostable DNA polymerase (green vials) Each tube contains all the reagents required to start with the amplification procedure. After the addition of the sample do not add polymerase or dNTPs! Before opening the tubes, lay them down on a transilluminator and expose the DNA amplicons to UV light for 60 seconds. The PCR fragments, UV activated, will not be amplified anymore. Open the tubes, and perform agarose electrophoresis
Interpretation of the results
Samples are considered positive when after electrophoresis a band of 700bp is detected.
Storage and stability
Twelve months at -20°C
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