Mycoplasma species Reagent Set

Code

EMK050050

Category

PCR Kit

Format

50rxns

Description

Reagent Set Mycoplasma

Mycoplasma species Reagent Set

Reagent Set for the detection of Mycoplasma in cell culture supernatant

Mycoplasma can easily contaminate cell cultures. Traditional detection methods (cultural and immunoenzymatic often do not have sufficient sensitivity to detect low grade contaminations) The primers are able to detect all Mycoplasma species and allow a high sensitivity

Starting sample

200µl of growing medium

Primers

Primers are aliquoted in single reaction tubes and isolated by paraffin (HOT-START) Primers contain an UV-activable DNA cross-linker that makes the amplicons not further suitable for PCR (post-amplification decontamination)

Reagents supplied

Sixty tubes containing 20µl of primer solution includes also dNTPs and Thermostable DNA polymerase (red vials). Two tubes containing 60µl of positive control including also dNTPs and Thermostable DNA polymerase (green vials) Each tube contains all the reagents required to start with the amplification procedure. After the addition of the sample do not add polymerase or dNTPs! Before opening the tubes, lay them down on a transilluminator and expose the DNA amplicons to UV light for 60 seconds. The PCR fragments, UV activated, will not be amplified anymore. Open the tubes, and perform agarose electrophoresis

Interpretation of the results

Samples are considered positive when after electrophoresis a band of 700bp is detected.

Storage and stability

Twelve months at -20°C